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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
22/05/2023 |
Actualizado : |
24/05/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
Autor : |
YANES, M.L.; FERNÁNDEZ, A.; ARIAS, A.; ALTIER, N. |
Afiliación : |
M. L. YANES, Instituto de Investigaciones Biológica Clemente Estable, Laboratorio de Ecología Microbiana, Montevideo, Uruguay; ALFREDO JOSÉ FERNÁNDEZ TECHERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; A. ARIAS, Instituto de Investigaciones Biológica Clemente Estable, Laboratorio de Ecología Microbiana, Montevideo, Uruguay; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Method to evaluate disease supression against Pythium debaryanum and alfalfa growth promotion by fluorescent Pseudomonas. [Método para evaluar protección contra Pythium debaryanum y promoción del crecimiento de alfalfa por Pseudomonas fluorescentes]. [Special Issue 25 Years Agrociencia]. |
Complemento del título : |
Plant Biology. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Agrociencia Uruguay, 2022, vol. 26, NE2, e1087. doi: https://doi.org/10.31285/AGRO.26.1087 -- OPEN ACCESS. |
ISSN : |
2730-5066 |
DOI : |
10.31285/AGRO.26.1087 |
Idioma : |
Inglés |
Notas : |
Article history: Article originally published in: Agrociencia (Uruguay). 2004;8(2):23-31. doi: https://doi.org/10.31285/AGRO.08.1002 -- Special Issue 25 Years Agrociencia. -- License: This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/ ) |
Contenido : |
ABSTRACT.- Fluorescent Pseudomonas have been extensively reported as effective biocontrol agents and can be used to manage Pythium seedling diseases in alfalfa. An in vivo methodology was developed to evaluate fluorescent Pseudomonas isolates for their ability to suppress disease and promote plant growth in the alfalfa-Pythium pathosystem. The standard method consists of a bioassay performed in growth chamber under controlled conditions of temperature and photoperiod. Alfalfa seeds were sown in commercial substrate and subsequently treated as follows: no Pseudomonas, no Pythium debaryanum (germination control); inoculated only with P. debaryanum (disease control); each one of the Pseudomonas isolates co-inoculated with P. debaryanum. An augmented experiment design was obtained by selecting a random complete blocks design for four checks systematically evaluated on 12 bioassays, and enlarging the blocks to accommodate up to 16 treatments per assay. Disease control recorded an emergence of 33.2% on day 15 after sowing date, as compared with germination control (100%). The 101 fluorescent Pseudomonas isolates showed a wide response on disease suppression ability. Twelve percent of tested isolates reasonably protected alfalfa plants against P. debaryanum, recording an emergence of over 60%. A similar procedure, in the absence of the pathogen, was used to evaluate alfalfa growth-promoting ability of selected Pseudomonas isolates. Five isolates exhibited a significant biocontrol activity against P. debaryanum and four of them also recorded a significant plant growth-promoting effect. Both bioassays were appropriate to identify candidate Pseudomonas isolates to be further tested under field conditions. Copyright (c) 2022 Agrociencia Uruguay MenosABSTRACT.- Fluorescent Pseudomonas have been extensively reported as effective biocontrol agents and can be used to manage Pythium seedling diseases in alfalfa. An in vivo methodology was developed to evaluate fluorescent Pseudomonas isolates for their ability to suppress disease and promote plant growth in the alfalfa-Pythium pathosystem. The standard method consists of a bioassay performed in growth chamber under controlled conditions of temperature and photoperiod. Alfalfa seeds were sown in commercial substrate and subsequently treated as follows: no Pseudomonas, no Pythium debaryanum (germination control); inoculated only with P. debaryanum (disease control); each one of the Pseudomonas isolates co-inoculated with P. debaryanum. An augmented experiment design was obtained by selecting a random complete blocks design for four checks systematically evaluated on 12 bioassays, and enlarging the blocks to accommodate up to 16 treatments per assay. Disease control recorded an emergence of 33.2% on day 15 after sowing date, as compared with germination control (100%). The 101 fluorescent Pseudomonas isolates showed a wide response on disease suppression ability. Twelve percent of tested isolates reasonably protected alfalfa plants against P. debaryanum, recording an emergence of over 60%. A similar procedure, in the absence of the pathogen, was used to evaluate alfalfa growth-promoting ability of selected Pseudomonas isolates. Five isolates exhibited a significant biocontrol a... Presentar Todo |
Palabras claves : |
Biocontrol; PGPR; Promoción del crecimiento vegetal; Pythium. |
Thesagro : |
CONTROL BIOLÓGICO; MEDICAGO SATIVA L; PSEUDOMONAS. |
Asunto categoría : |
H01 Protección de plantas - Aspectos generales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/17168/1/2730-5066-1087.pdf
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Marc : |
LEADER 03112naa a2200277 a 4500 001 1064157 005 2023-05-24 008 2022 bl uuuu u00u1 u #d 022 $a2730-5066 024 7 $a10.31285/AGRO.26.1087$2DOI 100 1 $aYANES, M.L. 245 $aMethod to evaluate disease supression against Pythium debaryanum and alfalfa growth promotion by fluorescent Pseudomonas. [Método para evaluar protección contra Pythium debaryanum y promoción del crecimiento de alfalfa por Pseudomonas fluorescentes]. [Special Issue 25 Years Agrociencia].$h[electronic resource] 260 $c2022 500 $aArticle history: Article originally published in: Agrociencia (Uruguay). 2004;8(2):23-31. doi: https://doi.org/10.31285/AGRO.08.1002 -- Special Issue 25 Years Agrociencia. -- License: This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/ ) 520 $aABSTRACT.- Fluorescent Pseudomonas have been extensively reported as effective biocontrol agents and can be used to manage Pythium seedling diseases in alfalfa. An in vivo methodology was developed to evaluate fluorescent Pseudomonas isolates for their ability to suppress disease and promote plant growth in the alfalfa-Pythium pathosystem. The standard method consists of a bioassay performed in growth chamber under controlled conditions of temperature and photoperiod. Alfalfa seeds were sown in commercial substrate and subsequently treated as follows: no Pseudomonas, no Pythium debaryanum (germination control); inoculated only with P. debaryanum (disease control); each one of the Pseudomonas isolates co-inoculated with P. debaryanum. An augmented experiment design was obtained by selecting a random complete blocks design for four checks systematically evaluated on 12 bioassays, and enlarging the blocks to accommodate up to 16 treatments per assay. Disease control recorded an emergence of 33.2% on day 15 after sowing date, as compared with germination control (100%). The 101 fluorescent Pseudomonas isolates showed a wide response on disease suppression ability. Twelve percent of tested isolates reasonably protected alfalfa plants against P. debaryanum, recording an emergence of over 60%. A similar procedure, in the absence of the pathogen, was used to evaluate alfalfa growth-promoting ability of selected Pseudomonas isolates. Five isolates exhibited a significant biocontrol activity against P. debaryanum and four of them also recorded a significant plant growth-promoting effect. Both bioassays were appropriate to identify candidate Pseudomonas isolates to be further tested under field conditions. Copyright (c) 2022 Agrociencia Uruguay 650 $aCONTROL BIOLÓGICO 650 $aMEDICAGO SATIVA L 650 $aPSEUDOMONAS 653 $aBiocontrol 653 $aPGPR 653 $aPromoción del crecimiento vegetal 653 $aPythium 700 1 $aFERNÁNDEZ, A. 700 1 $aARIAS, A. 700 1 $aALTIER, N. 773 $tAgrociencia Uruguay, 2022, vol. 26, NE2, e1087. doi: https://doi.org/10.31285/AGRO.26.1087 -- OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
22/12/2014 |
Actualizado : |
22/12/2014 |
Tipo de producción científica : |
Abstracts/Resúmenes |
Autor : |
MEDINA, M; VILARO, D.; CERETTA, S.; ABADIE, T. |
Afiliación : |
DIEGO CARLOS VILARO NIETO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SERGIO EDUARDO CERETTA SORIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Diseños experimentales eficientes para la caracterización del comportamiento agronòmico diferencial de cultivares de maíz para silo. |
Fecha de publicación : |
2000 |
Fuente / Imprenta : |
En: Seminario Panamericano de Semillas (17º, 2000, Punta del Este, Uruguay) Rueda de negocios y foro mundial sobre biotecnología y marketing de semillas. Resumenes y trabajos presentados. Punta del Este: Uruguay. INIA. |
Páginas : |
p. 104 |
Idioma : |
Español |
Thesagro : |
DISEÑOS EXPERIMIENTALES; MAIZ; URUGUAY. |
Asunto categoría : |
-- |
Marc : |
LEADER 00735nam a2200181 a 4500 001 1051932 005 2014-12-22 008 2000 bl uuuu u01u1 u #d 100 1 $aMEDINA, M 245 $aDiseños experimentales eficientes para la caracterización del comportamiento agronòmico diferencial de cultivares de maíz para silo. 260 $aEn: Seminario Panamericano de Semillas (17º, 2000, Punta del Este, Uruguay) Rueda de negocios y foro mundial sobre biotecnología y marketing de semillas. Resumenes y trabajos presentados. Punta del Este: Uruguay. INIA.$c2000 300 $ap. 104 650 $aDISEÑOS EXPERIMIENTALES 650 $aMAIZ 650 $aURUGUAY 700 1 $aVILARO, D. 700 1 $aCERETTA, S. 700 1 $aABADIE, T.
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